Enzymes are proteins that catalyze chemical reactions in biological systems in terms of the biocatalyze which has the nature of catalysts in general are involved react, but at the end of the reaction will be retrieved in its original form. Inhibitor is a compound that can inhibit or decrease the rate of reaction catalyzed by the enzyme. Enzyme inhibitors itself are divided into two kinds namely reversible and irreversible inhibitor. The aims of this experiment was to determine the effect of inhibitors on enzyme activity seen from the value of Vmax and KM reaction without inhibitor and with inhibitor. The method used was quantitative analysis. In this experiment were used sodium succinate as a substrate and sodium citrate as a inhibitor. The Na-citrate was inhibited reaction catalyzed by the enzyme succinate dehydrogenase. The value of KM in reaction without inhibitor is 6.754 x 10-2 M, while in reaction with inhibitor is 0.4 M. So, it can be conclude that addition of inhibitor concentration cause increasing in KM value but had no effect on the value of Vmax..Keywords: Enzyme, Succinate dehydrogenase, Competitive inhibitor
Redhana, I W. & Maryam, S. 2003. Penuntun Praktikum Biokimia. Singaraja: IKIP Negeri Singaraja.
Tika, I N. 2010. Buku Penuntun Praktikum Biokimia. Singaraja: Universitas Pendidikan Ganesha.
Tika, I N. 2007. Penuntun Praktikum Biokimia. Singaraja: Universitas Pendidikan Ganesha
Lakewood, 2011. Effects of Inhibitors on Enzyme Activity. Worthington: Biochemical Corporation.Sharma, Rakesh. 2012. Enzyme Inhibition and Bioapplications. India: InTech