Chromatography is introduced as a technique for separating and/or identifying the components in a mixture. One type of chromatography is a paper chromatography, which has the stationary phase and mobile phase that the liquid which is not mixed with each other. By using paper chromatography, it can be determined amino acid content that is existed in the sample by comparing the value of Rf sample components with the value of standard Rf. On paper chromatography experiment was used a mixture of n-butanol, aquades, and glacial acetic acid as the eluent. In this experiment, it was carried out a qualitative analysis of a solution containing several amino acids, which aims to determine the ratio of the distribution coefficient (Rf) from a variety of amino acids, especially glycine, cysteine, phenylalanine and tryptophan as well as determination on unknown sample by paper chromatography in ascending technique. Based on the experiment, the Rf value of glycine, cysteine, trypthopan, phenylalanine, unknown A, and B solutions namely 0.186; 0.197; 0.569; 0.605; 0.174; 0.232; 0.174; 0.558 and 0.581. Then, the amino acids that contained in the unknown A were glycine and cysteine. While, the amino acids that contained in the unknown B were glycine, tryptophan and phenylalanine.Keywords: Amino acid, Paper chromatography
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