Kamis, 16 Februari 2017

IDENTIFICATION OF AMINO ACID IN UNKNOWN SAMPLE THROUGH PAPER CHROMATOGRAPHY TECHNIQUE



Chromatography is introduced as a technique for separating and/or identifying the components in a mixture. One type of chromatography is a paper chromatography, which has the stationary phase and mobile phase that the liquid which is not mixed with each other. By using paper chromatography, it can be determined amino acid content that is existed in the sample by comparing the value of Rf sample components with the value of standard Rf. On paper chromatography experiment was used a mixture of n-butanol, aquades, and glacial acetic acid as the eluent. In this experiment, it was carried out a qualitative analysis of a solution containing several amino acids, which aims to determine the ratio of the distribution coefficient (Rf) from a variety of amino acids, especially glycine, cysteine, phenylalanine and tryptophan as well as determination on unknown sample by paper chromatography in ascending technique. Based on the experiment, the Rf value of glycine, cysteine, trypthopan, phenylalanine, unknown A, and B solutions namely 0.186; 0.197; 0.569; 0.605; 0.174; 0.232; 0.174; 0.558 and 0.581. Then, the amino acids that contained in the unknown A were glycine and cysteine. While, the amino acids that contained in the unknown B were glycine, tryptophan and phenylalanine.
Keywords: Amino acid, Paper chromatography



REFERENCES
Anonim. 2012. Paper Chromatography. Accessed on 13th March 2015 from www.chem-is-try.com
Purba, M. 2004. Kimia Untuk SMA Kelas XII. Jakarta: Erlangga
Redhana, I W. & Maryam, S. 2003. Penuntun Praktikum Biokimia. Singaraja: IKIP Negeri Singaraja
Tika, I Nyoman. 2010. Buku Penuntun Praktikum Biokimia. Singaraja: Universitas Pendidikan Ganesha
Voet, D. 2013. Fundamental of Biochemistry. America: John Willey and Sons, Inc.

Tidak ada komentar:

Posting Komentar