IDENTIFICATION OF AMINO ACID IN EGG ALBUMIN, UNKNOWN B AND D SOLUTIONS THROUGH MILLON, HOPKINS-COLE, NINHIDRIN, PbS, AND NITROPRUSIDE TESTS

Amino acid is a compound that is very important as a constituent unit of protein. Total amino acids exist in nature, only 20 types of amino acids contained in protein. The purposes of this experiment are to identify (1) the types of amino acids contained in protein solution (egg albumin) through Millon, Hopkins-Cole, Ninhydrin, PbS and Nitropruside test, and (2) the types of amino acids contained in the unknown sample through the amino acid test. The method used in this experiment was qualitative analysis. The result of this experiment were, first, egg albumin solution is positive in Millon, Hopkins-Cole, Ninhydrin, and PbS tests. It means that in egg albumin contains tyrosin, tryptophan, α-amino acid and sulfur. Second, unknown B solution is positive in Millon test, in which it contains hydroxyphenyl compound. Besides that, unknown B solution is also positive in Ninhydrin test in which it contains α-amino acids and peptides having free α-amino group. While, unknown D solution is positive in Nitropruside test, thus it contained –SH group. So, it can be concluded that egg albumin contains tyrosin, tryptophan, α-amino acid and sulfur; unknown B is tyrosine solution; and unknown D is cysteine solution.

Keywords: Amino acids, Egg albumin, Millon test, Hopkins-Cole test, Ninhydrine test, PbS test, Nitropruside test.

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